Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Protective effects of BM-MSCs on HPMVECs against oxygen-glucose deprivation (OGD). Phase contrast images of cultured HPMVECs with or without BM-MSCs: (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. (F) Administration of BM-MSCs alleviated OGD-induced high permeability in HPMVECs. This experiment was repeated three times in each group. Data are expressed as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Cell Culture, Permeability

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Effects of in vitro co-culture of OGD-treated HPMVECs with BM-MSCs on the mitochondrial membrane potential (ΔΨm). Red fluorescence indicates that JC-1 aggregates formed in cells with a high ΔΨm, whereas green fluorescence indicates that JC-1 monomers formed in cells with low ΔΨm. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: In Vitro, Co-Culture Assay, Fluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Effect of BM-MSCs on apoptosis and necrosis in OGD-induced HPMVECs. Cells were double-stained with Annexin V-FITC and PI and were then analysed using flow cytometry. This experiment was repeated independently three times in each group, and the representative results are shown. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+BM-MSCs group; (E) OGD+FIB group. (F) Statistical analysis of the proportions of early apoptosis and later apoptosis of HPMVECs. These results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Staining, Flow Cytometry

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: The autophagic levels in HPMVECs with or without BM-MSCs. Representative transmission electron microscopy (TEM) images for autophagy ultrastructures and quantitative analysis of the number of autophagosomes in different groups. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+BM-MSCs group; (E) OGD+FIB group. (F) Statistical analysis of the number of autophagosomes per μm 2 . Autophagosomes are indicated by arrows. N = cell nucleus. The results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Transmission Assay, Electron Microscopy

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: BM-MSCs co-culture increased the level of autophagosomes in OGD-induced HPMVECs. Green puncta revealed MDC-labelled autophagosomes. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Co-Culture Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: BM-MSC co-cultures increased the autophagy level in OGD-induced HPMVECs. The protein levels of LC3, p62, and β-actin were examined using Western blotting analyses. (A) Representative Western image of LC3-I, LC3-II, p62, and p-Akt in OGD-induced HPMVECs with or without BM-MSCs. (B–D) Statistical analysis of the expression of LC3-II/LC3-I, p62/β-actin, and p-Akt/Akt in OGD-induced HPMVECs. The results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Western Blot, Expressing